BOB

Open-Design Upright Microscope (Sutter Instrument)
Description

 

The Sutter BOB™ — designed to eliminate the conventional microscope frame — is a simple, open-design upright microscope platform ideal for slice electrophysiology, wide field functional imaging, two photon retinal imaging, photostimulation and new techniques just being developed! A microscope, in its simplest form, is an objective and a tube lens. Other components of most modern microscopes are designed to serve specific functions: different types of experiments, methods of illumination and means of signal detection.

Replacing the microscope frame with an optical rail builds in the ability to adjust the overall height of the microscope, unheard of in conventional microscope designs. Work on slices in January, do in vivo experiments in March. The BOB microscope is a compact, single assembly that mounts to the “blue rail” with one massive, stable connection. Focusing is motorized and incorporated between the focus arm and the optical rail.

Fluorescence epi-illumination is built into the basic BOB via an Olympus vertical illuminator. LED transmitted light illumination uses the Olympus Oblique Coherent Contrast (OCC) condenser. Sutter’s TLED and TLED controller form the trans-illumination light source. The TLED controller is capable of being triggered with a digital signal eliminating the need for shutters and adding the ability to photostimulate from the trans location. In experiments where transmitted light is not desired, the LED, condenser focus mechanism and OCC condenser are easily removed as a single assembly. Additionally, the transmitted light path is shorter than in other frames, allowing the microscope body to sit significantly lower than a conventional microscope. When the microscope is shorter, there is more stability, increased ergometrics and ease of use.

The Sutter BOB when configured with an optional motorized XY stage or translator with MPC-200 controller, takes full advantage of our free Multi-Link™ software program for micromanipulator positioning. During whole-cell patch recording in slices, it is common to search a large area of the slice to find appropriate neurons. If the BOB is configured with Multi-Link, after you find your target, Multi-Link will then retrieve your recording and stimulation pipettes to the same field of view so that you can begin recording immediately. If later you need to stimulate a region outside the current field of view, Multi-Link can release the recording pipette and allow you to reposition the objective and stimulating pipette(s) to the new stimulation region.

Applications

  • in vivo and in vitro
  • Whole-cell imaging
  • Intracellular imaging
  • Material science

 

FEATURES

  • Optional motorized fixed XY stage or motorized translator
  • Open design microscope with motorized focus
  • Quickly configurable based on experimental needs
  • Optimized to allow in vivo and in vitro experimentation on one setup
  • Designed for use with Olympus objective lenses
  • Free Multi-Link™ software coordinates movement with micropipette positioning of MPC-200
  • Oblique Coherent Contrast (OCC) or Differential Interference Contrast (DIC)
  • Epi-fluorescent illumination

 

OPTIONS

BOB systems with fluorescence

BOB-FL   BOB microscope with epi-illuminator

BOB-FLTL   BOB with epi-illuminator, transmitted light

BOB-FLTL2   BOB with epi-illuminator, dual transmitted light

BOB-FLTR   BOB with epi-illuminator, trinocular head

BOB-FLTRTL   BOB with epi-illuminator, trinocular head, transmitted light

BOB-FLTRTL2   BOB with epi-illuminator, trinocular head, dual transmitted light

BOB systems without fluorescence

BOB   basic BOB microscope system

BOB-TL   BOB with transmitted light

BOB-TL2   BOB with dual transmitted light

BOB-TR   BOB with trinocular head

BOB-TRTL   BOB with trinocular head, transmitted light

BOB-TRTL2   BOB with trinocular head, dual transmitted light

 

OBJECTIVES

The BOB uses a standard Olympus 2-position nosepiece. You can use your own objectives or select up to 2 from the choices below.

OBJ-522     4X-UPLFLN4X; U PLAN FLUORITE 4× OBJECTIVE; NA 0.13, WD 17 mm  (recommended for electrophysiology)

OBJ-342     5X-LMPLFLN5X; LWD M PLAN FL 5× OBJECTIVE; NA 0.13, WD 22.5 mm

OBJ-343     10X-LMPLFLN10X; LWD M PLAN FL 10× OBJECTIVE; NA 0.25, WD 21 mm

OBJ-524     10X-UPLFLN10X2; U PLAN FLUORITE 10× OBJECTIVE; NA 0.3, WD 10 mm  (recommended for electrophysiology)

OBJ-824     10X-UPLSAPO10X2; U PLANS S-APO 10× OBJECTIVE; NA 0.4, WD 3.1 mm

OBJ-525     20X-UPLFLN20X; U PLAN FLUORITE 20× OBJECTIVE; NA 0.5, WD 2.1 mm

OBJ-585     20X-UMPLFLN20XW; LWD PLAN FLUOR 20× WTR DIPPING OBJ; NA 0.5, WD 3.5 mm

OBJ-766     20X-UAPON20XW340; UAPON 20× WATER 340 nm OBJ; NA 0.7, WD 0.35 mm

OBJ-825     20X-UPLSAPO20X; U PLAN S-APO 20× OBJECTIVE; NA 0.75, WD 0.6 mm

OBJ-527     40X-UPLFLN40X; U PLAN FLUORITE 40× OBJECTIVE; NA 0.75, WD 0.51 mm

OBJ-587     40X-LUMPLFLN40X/W; U M PLAN FLN 40×/W OBJ; NA 0.8, WD 3.3 mm, IR, ECO  (recommended for electrophysiology)

OBJ-769     40X-UAPON40XW340; UAPON 40× WATER OBJ; NA 1.15, WD 0.25 mm, W/CC, BFP1

OBJ-350     50X-LMPLFLN50X; LWD M PLAN FL 50× OBJECTIVE; NA 0.5, WD 10.6 mm

OBJ-592     60X-LUMPLFLN60X/W; U M PLAN FLN 60×/W OBJ; NA 1.0, WD 2.0 mm, IR, ECO  (recommended for electrophysiology)

OBJ-672     60X-LUMFLN60X/W; NA 1.1, WD 1.5 mm, 25 ANGLE, IR.CC, ECO  (recommended for electrophysiology)

OBJ-832     60X-UPLSAPO60XO; U PLAN S-APO 60× OIL OBJ; NA 1.35, WD 0.15 mm, BFP

OBJ-893     60X-UPLSAPO60XW; U PLAN S-APO 60× W OBJ; NA 1.2, WD 0.28 mm, W/CC 0.13-0.21

 

CONDENSORS

For models with TL in the part number, choose a condenser. For DIC models, select the condenser that matches the highest power of your selected objectives.

OCC     Oblique coherent condenser

DIC-40X     DIC condenser for BOB with 40× objective

DIC-40XIR     DIC condenser for BOB with 40× objective and IR

DIC-60X     DIC condenser for BOB with 60× objective

DIC-60XIR     DIC condenser for BOB with 60× objective and IR
         

FILTER CUBES

For models with FL in the part number, choose a filter cube(s) and a light source.

CUBE-GFP     Filter Cube - GFP

CUBE-CY3     Filter Cube - CY3

CUBE-YFP     Filter Cube - YFP

CUBE-DAPI     Filter Cube - DAPI

CUBE-A635     Filter Cube - ALEXA 635

Many more filter cubes available on request.

 

LIGHT SOURCES

HPX-L5     Lambda HPX-L5 High powered LED light source

LS-OF30     Lambda LS with 300 Watt ozone free lamp

LB-421     Lambda 421 Optical Beam Combiner and controller

LB-OBC     Lambda OBC Optical Beam Combiner

FLED-BOB     Lambda FLED for BOB

FLED-DC-BOB     Dual Channel Lambda FLED for BOB

LG-BOB     Light guide adapter

 

ACCESSORIES

Moving Platforms
(Please specify chamber type when ordering)

MPC-88     Moving platform stage plate with motorized gantry supports, MPC-200 controller and ROE, cables and manual
MPC-88/M6     Same as MPC-88 except with M6 tapped holes

MPB-88     Moving platform stage plate with motorized gantry supports, BOB controller, cables and manual
MPB-88-M6     Same as MPB-88 except with M6 tapped holes

MP-88     Moving platform stage plate with motorized gantry supports, MP-285 controller and ROE, cables and manual
MP-88/M6     Same as MP-88 except with M6 tapped holes

common MP-88 / MPC-88 Chamber inserts
(no charge when ordered with MP-88 or MPC-88)

X040500     Warner Series 20 (round) - aluminum
X040503     Warner Series 20 (round) - stainless steel
X040505     Warner Series 20 (octagonal) - aluminum
X040507     Warner Series 20 (octagonal) - stainless steel
X040510     50 mm round - aluminum
X040512     50 mm round - stainless steel
X040497     Blank insert - Aluminum
X040498     Blank insert - stainless Steel
SI-SLIDE     Insert with slide holder
SI-W30       Warner Series 30 platform - aluminum
SI-W30ST     Warner Series 30 platform - stainless steel
SI-ALA-MS     ALA MS series insert - aluminum

other MP-88 / MPC-88 Chamber inserts

SI-PETRI        for Petri dish and slides
SI-CUSTOM     custom stage insert

 

Motorized Translator and Stands

MT-2000-BOB     Motorized translator with MP-285 controller, ROE, and stands

MT-2200-BOB     Motorized translator with MPC-200 controller, ROE, and stands

MT-2800-BOB     Motorized translator with BOB controller, ROE, and stands

Motorized Translator and Fixed Platform Stage

MT-2088-BOB     Motorized translator with MP-285 controller, ROE, and fixed platform stage

MT-2288-BOB     Motorized translator with MPC-200 controller, ROE, and fixed platform stage

MT-2888-BOB     Motorized translator with BOB controller, ROE, and fixed platform stage

Motorized Translator only  (no stands or platform)

MT-800-BOB     Motorized translator with MP-285 controller and ROE

MT-820-BOB     Motorized translator with MPC-200 controller and ROE

MT-880-BOB     Motorized translator with BOB controller and ROE

 

Camera

CAM-EL     ELECTRO, Cooled 1.4 MP CCD Camera

CAM-PR     PRIME 25B, Back illuminated CMOS Camera

CAM-BSI     PRIME BSI, High-res scientific CMOS Camera