Dagan PC ONE
- Can do Patch or Whole Cell with optional Dual Range
- PC-ONE-60 Remote Switchable Headstage
- 3 ½ Digit LCD Panel Meter
- Telegraphs for GAIN and FILTER
- 10 MΩ Headstage for Large Whole Cells
- Auto Zero with SEARCH and HOLD capability
- Unfiltered and Filtered Im Output
- Low Noise, High Speed
- Built in Leakage Subtraction
- Popular 50:1 and 10:1 Command Inputs
- Voltage/Current or Zero Current Mode from External Control
- Five position 3-pole Low Pass Bessel Filter
- Offsets up to ± 300 mV
- Two Value Test Generator for pipette and seal resistance
- Eight Output Gains
The PC-ONE accepts SEVEN types of Headstages to give you the EXACT Headstage for your Application:
10 MΩ Large Whole Cell (to 1000 nA) (Model # PC-ONE-10) 100 MΩ Whole Cell (to 100 nA) (Model # PC-ONE-20) 1 GΩ Small Whole Cell (to 10 nA) (Model # PC-ONE-30) 10 GΩ Patch (to 1 nA) (Model # PC-ONE-40) * 10 GΩ Bilayer (Model # PC-ONE-50) 100 MΩ /10 GΩ Dual Range Whole Cell/Patch (Model # PC-ONE-60) 2 V Command for VOLTAMMETRY (10 MΩ through 10 GΩ) (Model # PC-ONE-70)
* PC-ONE-40 is supplied if no headstage is specified
CELL ATTACHED (SINGLE CHANNEL) PATCH CLAMPING - The 10 GΩ standard Headstage (PC-ONE-40) or the optional Dual Range Headstage (PC-ONE-60) allow for low noise (0.30 pA rms) recording. Ease of use was paramount with the PC-ONE design team. The PC-ONE is equipped with the standard "Auto Zero" or HOLD Junction Potential Correction as well as a continuous SEARCH capability. The FILTER has five ranges suitable for all modes of operation.
WHOLE CELL VOLTAGE AND CURRENT CLAMPING - The PC-ONE handles three ranges of whole cell headstages for currents up to 1000 nA (10 MΩ feedback). Standard LEAKAGE subtraction/compensation allows you to quickly adjust for leak errors on-line during an experiment.
VOLTAMMETRY (ELECTROCHEMICAL) APPLICATIONS - A special headstage with a ±2 volt command range allows the PC-ONE to "voltage clamp" a carbon-fiber electrode so that extracellular concentrations of various neurotransmitters can be measured at any specified oxidation/reduction potential. Headstages can be specified with 10 MΩ, 100 MΩ, 1 GΩ, or 10 GΩ feedback sensing resistors (i.e., 1000 nA down to 1 nA full scale sensitivities).
PLANAR LIPID BILAYER RECORDING - A special Bilayer headstage (10 GΩ feedback resistor) not only allows applications to 500 pF Bilayers, but it also allows you to "short" the Bilayer during the onset of a step command to "quick charge" the Bilayer capacitance. A unique 10 GΩ (Bilayer) position on the Headstage Selector switch modifies the Capacitance Compensation from the normal 10 pF Patch application to the 500 pF Bilayer application.
EASY MODE CONTROL - Selectable Voltage Clamping and both Current Clamping with Command or Zero Command (protects cells when changing modes). A connector on the rear panel allows external computer control of modes. Two LED's indicate the mode selected.
EASY COMPUTER INTERFACING - The PC-ONE rear panel has a 15-pin connector that easily unplugs, breaking Input/Output connections (optional I/O cable). Thus if desired Im, FILTERED Im, Vm and VTOT can be disconnected very conveniently. The rear panel also contains the headstage connector, chassis/circuit ground jacks, Buz-1, Speed Test Input BNC, Test SYNC BNC, Gain and Filter Telegraph BNC'S, and the power cord interlock.
Noise: (PC-ONE-40 10 GΩ headstage)
1.0 kHz Bandwidth, 0.06 pA rms; 3.0 kHz Bandwidth, 0.10 pA rms;
5.0 kHz Bandwidth, 0.16 pA rms; 10 kHz Bandwidth, 0.30 pA rms.
Fast: 0 to 1 µs Slow: 0 to 1 ms (Patch)
Range: 10 pF Patch; 100 pF Whole Cell; 500 pF Bilayer
Series Resistance Compensation:
0 to 100 MΩ, ten turn dial (all whole cell ranges)
Filter: 100, 300, 1k, 3k, 10 kHz Low Pass Bessel Filter (3 pole)
Gain: 0.1, 0.2, 0.5, 1, 2, 5, 10, 20 amplification (b) of Im signal with headstage scale factor (a) of 0.1, 1, 10, 100 for sensitivities up to 2000 mV/pA (Patch) (Scale factor (a) is shown on convenient LED's next to the GAIN switch)
Junction Potential Correction:
(3 types, all +300 mV) Manual-ten turn control; Search continuously corrects potential with a slow "attack" time; Hold correction potential only when UPDATE switch is pushed
TEST switch with 0.2 mV and 20 mV positions for pipette and seal resistance forming seal (2 pA and 200 pA Current Clamp)
Holding Potential Command:
+200 mV ten turn control with ON/OFF switch (+2 nA Current Clamp)
3 ½ digit LCD with 5 functions and automatic decimal point:
- COMD IN - the command at the command input (1999 mV)
- VHOLD - the holding potential (1999 mV)
- VTOT - the total of all commands presented to the pipette (1999 mv)
- VM - the membrane voltage (1999 mV)
- IM - the membrane current before processing:
199.9 pA 10 GΩ headstage; 19.99 pA 100 MΩ headstage
Step Command Input:
50 : 1 (i.e., 10 V gives 200 mV) industry standard, and 10 : 1 with center OFF (10 kΩ input, 10 V max) (5 V/nA or 1 V/nA Current Clamp)
High Frequency Boost:
Automatic with no need for confusing ON/OFF switch
Computer Telegraph BNC Outputs:
GAIN and FILTER, analog 0.4 V per step
External Mode Select:
From Izero Mode, 5 V CMOS input allows either Vclamp or Iclamp selection via I/0 15-pin connector
Membrane Current (Im) Output:
Im: Unfiltered, Im signal. Sensitivity=(a)(b)mV/pA (used to record data unfiltered)
FILTERED Im: Filtered lm signal.
Sensitivity = (a)(b)mV/pA (used to view data)
Sample & Hold Option:
TTL activated Sample & Hold on Im path via back panel BNC
Electrode Holders: (one supplied)
Low noise polycarbonate with 1mm pressure port and BNC sized (i.e., industry standard) end. Specify for glass size (HB180 is standard): HB120 accepts glass 1.0 to 1.2 mm OD; HB180 accepts glass 1.5 to 1.8 mm OD (silver wire internal). Electrode holders in other designs and sizes are available too.
7.7 cm × 3.5 cm × 2.8 cm; detachable mounting rod is 0.62 cm diameter by 7.6 cm long. Cable length is 1.8 m
9 cm high, 48 cm wide, 25 cm deep. Supplied with rack mounting hardware
Power: 100/115 or 230 V / AC 50/60 Hz (15 W)
Delivery subject to the reservation of limited or no availability from the manufacturer.