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The Lambda
DG-4 offers unprecedented speed and
versatility for experiments requiring
rapid light wavelength switching. It
offers all the advantages of interference
filter-based systems, yet eliminates the
temporal constraints imposed by filter
switching devices. Switching between any
two wavelengths is achieved in less than
the 1.2 msec vertical retrace period of a
video signal, allowing you to perform
real-time video imaging. For dual
wavelength ratio imaging studies, the
Lambda DG-4 enhances your ability to
follow fast changes in ion concentrations
by acquiring a ratio pair in two
consecutive video frames.
Narrow
bandpass systems, such as single cavity
interference filters, grating
monochromators, and A.O. modulators, will,
by their nature, pass harmonics of the
desired wavelength. With variable
wavelength devices, it is not always
possible to obtain sufficient blocking of
out-of-band wavelengths. Modern
interference filters, as used in the
Lambda DG-4, have integral blocking
characteristics 1000 times better than
typical monochromator systems. In
addition, it is difficult to adjust the
intensity of one wavelength relative to
another in a traditional system with a
single optical path and a variable
wavelength device. The dual galvanometer
design of the Lambda DG-4 eliminates this
problem.
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HOW
IT WORKS
This unique optical design of the Lambda DG-4, is
based on dual scanning galvanometers. The wavelength
selection is done by interference
filters. The standard version of the instrument, the
Lambda DG-4,
can host up to four 25mm interference filters.
A five filter version (two 25mm and three
18mm), Lambda DG-5 is also available. The light coming
from the 175W xenon arc lamp is focused on the first
galvanometer mirror.
The light is then directed, via a parabolic
mirror, through one of the optical channels that may
contain an interference filter. The light passing
through the filter is
collected by another parabolic mirror and sent to a second
scanning mirror that directs it to a
liquid light guide. The light guide can be
coupled to the illumination port of an instrument
(e.g. epi-illumination port of a microscope).
The intensity of the output can be modulated by
controlling the relative orientation of the two
scanning mirrors. Thus this system can provide
narrow band excitation at selected wavelengths
over a range of intensities or can rapidly turn off
the light source. Dwell time at any wave-length is
arbitrarily set by the user. Transitions are achieved
in less than 1.2msec.
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