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The SF-77B is a simple but
highly effective stimulus solution delivery
device for use in a variety of patch clamp and
electrophysiology studies. Control and test
solutions flow continuously through adjacent
stimulus delivery tubes and a stepper mechanism
selects which tube is directed at the
preparation. The rapid response and nominal
hysteresis of the stepper mechanism allows for
very fast switching times. Complete solution
changes are typically achieved within 20 msec
for a large 700 mm step and times are
significantly shortened as the step size is
decreased.
Multiple
Solution Studies
In the standard configuration, up to six
different solutions are connected to a single
input manifold which in turn is connected to one
of three square glass stimulus ports. The three
stimulus port design is superior to a two-port
design in that complex solution exchange
protocols can be brought to bear on the sample
under study. Since the complete system is
designed to accommodate three manifolds (one for
each stimulus port) and each manifold can
accommodate up to 6 feed lines, it is possible
to immediately select between 18 different input
solutions.
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Solution
changes between stimulus ports occur within
milliseconds
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Changes
between solutions connected to individual
port occur within 5 secs
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Entirely
new solutions can be added into any port
with a waiting time of no more than 30
seconds
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Cell
is never required to pass through
intervening solutions to get from control to
test solution
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Manual
or External Control
The stepper mechanism can be manually controlled
via the front panel or externally directed from
your data acquisition program. Manually, the
system can be stepped to 8 positions in 7
equally spaced steps. These same 8 positions can
also be directly selected by applying an analog
signal to the external analog input BNC or by
passing a 3 byte word to the TTL input on the
instrument rear panel.
Square
Glass Stimulus Ports
The square glass tubes used for solution
delivery significantly reduces mixing turbulence
between solution flows allowing the SF-77B
to be used for studies with both membrane
patches and whole cells, even when the cells are
not fixed to a substrate.
System
Versatility
The design of the SF-77B permits the use of
various size glass tubing for perfusion
delivery.
SF-77B
Standard System
(0.7 mm ID tubes): This standard system is
shipped with 3SG700-5 single-walled 3-barrel
glass tubing which eliminates the need to glue
individual barrels together. Spacing between
barrels is 0.7 mm and step speed between
adjacent barrels is typically 20 msec. Single
barrel SG800-5 tubes (up to 5) can be used with
the same holder.
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Number
of Steps
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1
to 7 (2 to 8 positions)
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Step
Size
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Adjustable
from 100 µm to 1.5 mm in 100 µm
increments, with STEPS/POSITION switch
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Step
Speed
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Typically
20 msec for 700 µm step
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Step
Control
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Manual
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8
positions with POSITION selector
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Analog
Signal
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5
positions with voltage levels 0-4 V, 1V
per step
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Digital
Signal
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8
positions with 3 byte TTL signal
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Max.
Stepped Range
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12.5
mm
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Mounting
Handle
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6.3
mm X 10 cm (D x L)
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Stepper
Weight
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110.5
g (including handle)
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Solution
Manifolds
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Three
manifolds supplied
MM series for SF-77B and SF-77BST
ML series with SF-77 BLT
Manifolds have 2, 4 or 6 inputs
User specifies when ordering
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MM
Series
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MM-2,
MM-4 and MM-6 manifolds use PE-50 tubing
at input and PE-10 tubing at output
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ML
Series
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ML-2,
ML-4 and ML-6 manifolds use PE-50 tubing
at both input and output
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Solution
Flow Rates
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Rates
measured with solution reservoir height
of approx. 60 cm (24 in)
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With
MM Series
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100
µL/min
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With
ML Series
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1
mL/min
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Control
Box
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Size,
H x W x D
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6.3
x 14 x 23 cm
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Power
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100
to 130 or 220 to 250 VAC, 50/60 Hz, 10
VA
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Shipping
Weight
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2.7
kg
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Warranty
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2
years, parts and labor
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SF-77B/5M
Standard System with 5 tubes
(0.7 mm ID tubes): This is the same package as the
standard system except that the manifold bracket
and tube holder have been extended to accept 5
single tubes and associated perfusion lines. The
individual tubes can be glued together to make a
larger assembly, if desired. Spacing between
barrels is 0.6 mm and step speed between adjacent
barrels is typically less than 20 msec. Also
included are four (4) 6-to-1 MM Series manifolds
and one (1) 2-to-1 MM Series manifold.
SF-77BLT:
Large Tube System
(1.0 mm ID tubes) Larger stimulus ports are
required when using the SF-77B
with larger cell structures such as the Xenopus
oocyte. Solutions are delivered through 1.0 mm ID
square tubes (SG1000-5) with barrel-to-barrel
spacing of 1.4 mm.
SF-77BST:
1 msec Stepping With Theta Tubing
The SF-77B has been successfully
tested using theta style capillary tubing with a
step speed of 1 msec between adjacent barrels.
Standard 2 mm diameter theta tubing is pulled to a
tip diameter of 300 um with a spacing of 100 um
between barrels. In this design, placement of the
pipette tip is critical due to the smaller
perfusion stream. Since mechanical artifacts can
be evident when using these small step sizes the
motor drive voltage is adjustable at the rear
panel.
Easy
Set-Up
The stepper mechanism is compact, lightweight, and
free of either mechanical or electrical noise. The
mechanism connects to the control box with a 2
meter shielded cable and is provided with a
mounting rod for attachment to a manipulator.
Manifolds can support 2,4 or 6 inputs depending on
the experiment. Solutions flow from reservoirs to
the manifold through PE-50 tubing and PE-10 tubing
is used to connect the manifold outputs to the
glass tubes.
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