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Unique
features
Fast and automated solution change
No dead volume
No cross contamination between solutions
Precise setting of the flow with optional
programmable
motorized syringes
Compatible with Axon and Heka software
Introduction
on RSC operation
Change
of concentration of ion, neurotransmitters or of all
possible sort of ligands to be tested is an important
issue in modern patch-clamp recordings. The Bio-Logic
Rapid Solution Changers are the simplest and
easiest-to-use systems to achieve this goal.
These
instruments use the principle known as "sewer
pipes" in which the cell or the tip of the
electrode (in excised-patch mode) is positioned close
to the output of a capillary. The solution to be
assayed is flowing out of the capillary at a moderate
flow rate. The cell, being exposed in this stream,
very rapidly equilibrates in the perfusion solution.
The
fact that all solutions are flowing in individual
tubes right to their end eliminates all problems of
dead volumes, washing and cross-contaminations between
solutions to be assayed (1,2). This perfusion
principle has been used in many laboratories where it
has been proved that it was a very adequate system for
solution assays.
Fast
and automated solution change :
The
solutions to be assayed are driven to the rotating
head of the RSC. The solution exchange is performed by
a very precise rotation of the RSC head which exposes
the patch pipette or the cell to the flow of one of
the tubes. Times of rotation from one tube to the
adjacent one can be programmed from 2 to 800 ms. The
motor is light and vibration free thanks to the disk
rotor technology and to the micro step command
delivered by the controller. Due to the high number of
tubes it is possible to have one tube for every
solution to be assayed. As compared to fixed tube
devices, the rotating head permits a fast change of
the perfusion solution around the patch pipette
without any contamination from the previous solution
at the time of solution switching.
Comparison
between RSC-160 & RSC-200 |
1.
Command box;
2. Stepping motor;
3.
Rotating head (4 to 36 holes); 4.
Set of tubing
5. Set of syringes; 6.
Micromanipulator
7.
Magnetic stand; 8. Rack with 9 electrovalves
9.
Syringe rack holder
Command
box
Rotating
straight head
(9 holes)
Ordering
information |
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RSC-160
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RSC-200
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Tube
control
|
up
to 9
|
up
to 36
|
|
Valve
or motorized syringes control (with optional
EVH-9 or MS-x Unit(s))
|
up
to 9 (with 1 x EVH-9 or MS-1 + MS-8)
|
up
to 36 (with 4 x EVH-9 or 4 x [MS-1 + MS-8])
|
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Built-in
stimulator
|
No
|
Yes
|
|
GPIB
interface
|
No
|
Yes
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All
other specifications are the same for the two
instruments
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The
RSC-160 & RSC-200 are both delivered complete
with: command box, stepping motor, driving software,
one syringe rack with 18mm holes (or 32mm on request)
and one "Straight head" (and a tubing kit).
All other parts listed on Specifications site are
optional and should be specified on order or may be
purchased separately.
The
straight head is delivered with a tubing kit
composed of: 100 x Glass
capillaries of an outside diameter 1.00mm (Ref: GB
100TF 8P - thin wall with filament, ID=0.78 mm,
length: 8cm, fire polished ends); Polyethylene
tubing (5 meter length, ID: 0.3, OD: 0.70mm);
C-Flex tubing (7,5 meter length, ID: 0.5mm, OD: 2.1mm);
9 x luer lock female; 9
x 10ml plastic syringe
(or 50ml syringe on request);2
x O-Rings |
