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RC-10;
RC-11
, RC-13 & RC-16
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Small
Volume Imaging Chamber RC-10 (160 µl)
and
Large Volume Imaging Chambers RC-11
(190ul), RC-13 (260ul) & RC-16 (400ul)
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Warner
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The
RC Series are open bath chambers
suitable for physiological experiments performed
with inverted or dissecting microscopes.
Relevant procedures include patch clamp,
recording from oocytes, isolated perfused tubule,
and cell impalement. They have also been used
for perfusion in fluorescence microscopy. RC
Series chambers are made from polycarbonate.
A 24 x 60 mm, #2 thickness coverslip (user
attached) becomes the floor of the bath. The
coverslip is attached with vacuum grease or more
permanently with Sylgard or RTV cement.
Perfusion can be gravity fed and removed via
suction, or alternatively, input and output can
be pump controlled. The perfusion input accepts
standard 1.57 mm OD (PE-160) polyethylene tubing.
Solutions are removed from a separate suction
reservoir to minimize noise in the recording.
The suction tube is adjustable to control the
bath solution level. Flow rates can exceed 10
ml/min with gravity feed. Multiple perfusion
lines can be connected using one of the MP
manifolds. The MP manifold replaces the single
input perfusion connector.
An isolated reservoir is provided for an agar
bridge in the recording ground circuit. An
additional agar bridge well (for oocyte
recording) can be specified at no additional
cost.
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RC-10
This
small bath model, 10 mm in diameter, is
recommended where smaller volumes and fast
exchange times are important.
Bath
volume is 160 µl with 2 mm solution height. A
single agar bridge well is supplied on this
model.
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RC-11,
RC-13 & RC16
These
larger bath chambers are used for studies of
oocytes, slices and other larger
preparations.
Models RC-13 and RC-16 may also be used in patch
clamp experiments where cultured coverslips
are placed in the bath. RC-13 accepts 12 mm
diameter coverslips and RC-16 accepts 15 mm
diameter coverslips. A groove is milled in the
chamber underside to hold the coverslip in place.
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Note:
The double thickness of glass (bottom plus
sample coverslips) may not be compatible with
some short working distance microscope optics.
Mounting
the chamber
RC
Series chambers are mounted to a microscope with
one of the RC Series stage adapters.
Ordering
Information
Each chamber is supplied with: 1
plug-in perfusion input manifold [single input];
1 Pkg (40) #2 coverslips, 24 x 60 mm; 2
glass and 1 stainless steel suction tubes
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Imaging
Chambers
Products
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