RC-28

Excised Patch Chamber

Warner

   

      

 

 

 

 

 

This chamber is designed for electrophysiological recordings of excised patches. The dual bath construction permits exchange of the solution surrounding the membrane patch without contamination of the entire population. The chamber consists of two independent bath compartments separated by a dam/reservoir which functions as a gate and barrier to prevent mixture of the solutions. The composition and exchange rate of each bath can be controlled independently.


After patching onto a cell in the larger bath compartment, the dam/reservoir is flooded with solution establishing a "solution bridge" between the two compartments. This enables secure transfer of the electrode attached cell to the smaller compartment. The dam/reservoir is then emptied by vacuum suction thus separating the two baths.

This model is used with either P-6 or PH-6 (heater) platform. 

Specifications 

Material

Polycarbonate

Small Bath Dimensions

Length x Width

28 x 3.8 mm

Specimen Area

20 x 3.8 mm

Volume/mm of solution height

90 µl

Large Bath Dimensions

Length x Width

28 x 3.8 mm

Specimen Area

21 x 9.5 mm

Volume/mm of solution height

235 µl

Standard Accessories Supplied

PE-160 Tubing (10')

24 x 50 mm #1 Coverslip (pkg. of 50)

Platforms Used With

P-6 Plain Platform

PH-6 Heating Platform

 
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