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The
RC-1Z chamber features a unique
slot-shaped bath for applications requiring
rapid perfusion.
The
chamber is constructed using a two-piece design.
The top piece forms the chamber body and
contains a solution aspiration reservoir. An
agar bridge well with connecting channel to the
input side of the bath is also provided. An
isolated well is provided on the downstream side
of the bath, however, most users place the sense
electrode directly into the suction well.
The
bottom piece forms the floor of the chamber and
houses the oocyte work area. To aid in securing
the oocyte into position during impalement, the
chamber bottom has three dimples with diameters
of 0.8, 0.9, and 1.0 mm. Three grooves milled
into the chamber bottom (parallel to the
direction of solution flow) aid in bathing the
bottom side of the oocyte during perfusion.
Solutions
are removed from the suction well via an
adjustable suction tube and bath solution height
is adjusted by raising or lowering the suction
tube. The perfusion input accepts standard
1/16" OD (PE-160) polyethylene tubing.
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Material
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polycarbonate
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Footprint
(L x W)
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7.7
x 5.1 cm
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Bath
Dimensions (L x W x H)
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25
x 3.5 x 2.5 mm
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Volume,
by depth
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85
μl/mm
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Chamber
Floor Insert
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25
x 75 x 3 mm, polycarbonate
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Input
Tubing (ID x OD)
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1.14
x 1.57 mm (PE-160)
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Aspirator
coupling
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1.67
mm (OD)
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Specially designed for oocyte studies