PC- One

	PC-ONE
	Patch/Whole Cell Clamp Amplifier	Dagan

	FEATURES Can do Patch or Whole Cell with optional Dual Range PC-ONE-60 Remote Switchable Headstage 3 1/2 Digit LCD Panel Meter Telegraphs for GAIN and FILTER 10 MOhm Headstage for Large Whole Cells Auto Zero with SEARCH and HOLD capability Unfiltered and Filtered Im Outputs Low Noise, High Speed Built in Leakage Subtraction Popular 50:1 and 10:1 Command Inputs Voltage/Current or Zero Current Mode from External Control Five position 3-pole Low Pass Bessel Filter Offsets up to ±300mV Two Value Test Generator for pipette and seal resistance Eight Output Gains
	The PC-ONE accepts SEVEN types of Headstages to give you the EXACT Headstage for your Application: 10 MOhm Large Whole Cell (to 1000nA) (Model # PC-ONE-10) 100MOhm Whole Cell (to 100nA) (Model # PC-ONE-20) 1GOhm Small Whole Cell (to 10nA) (Model # PC-ONE-30) 10GOhm Patch (to InA) (Model # PC-ONE-40) * 10GOhm Bilayer (Model # PC-ONE-50) 100MOhm /10GOhm Dual Range Whole Cell/Patch (Model # PC-ONE-60) 2 volt Command for VOLTAMMETRY (10MOhm through 10GOhm ) (Model # PC-ONE-70) * PC-ONE-40 is supplied if no headstage is specified CELL ATTACHED (SINGLE CHANNEL) PATCH CLAMPING - The 10GOhm standard Headstage (PC-ONE-40) or the optional Dual Range Headstage (PC-ONE-60) allow for low noise (0.30pA rms) recording. Ease of use was paramount with the PC-ONE design team. The PC-ONE is equipped with the standard "Auto Zero" or HOLD Junction Potential Correction as well as a continuous SEARCH capability. The FILTER has five ranges suitable for all modes of operation. WHOLE CELL VOLTAGE AND CURRENT CLAMPING - The PC-ONE handles three ranges of whole cell headstages for currents up to 1000nA (10MOhm feedback). Standard LEAKAGE subtraction/compensation allows you to quickly adjust for leak errors on-line during an experiment. VOLTAMMETRY (ELECTROCHEMICAL) APPLICATIONS - A special headstage with a ±2 volt command range allows the PC-ONE to "voltage clamp" a carbon-fiber electrode so that extracellular concentrations of various neurotransmitters can be measured at any specified oxidation/reduction potential. Headstages can be specified with 10MOhm , 100MOhm , 1GOhm or 10GOhm feedback sensing resistors (i.e., 1000nA down to 1nA full scale sensitivities). PLANAR LIPID BILAYER RECORDING - A special Bilayer headstage (10GOhm feedback resistor) not only allows applications to 500pF Bilayers, but it also allows you to "short" the Bilayer during the onset of a step command to "quick charge" the Bilayer capacitance. A unique 10GOhm (Bilayer) position on the Headstage Selector switch modifies the Capacitance Compensation from the normal 10pF Patch application to the 500pF Bilayer application. EASY MODE CONTROL - Selectable Voltage Clamping and both Current Clamping with Command or Zero Command (protects cells when changing modes). A connector on the rear panel allows external computer control of modes. Two LED's indicate the mode selected. EASY COMPUTER INTERFACING - The PC-ONE rear panel has a 15-pin connector that easily unplugs, breaking Input/Output connections (optional I/O cable). Thus if desired Im, FILTERED Im, Vm and V_TOT can be disconnected very conveniently. The rear panel also contains the headstage connector, chassis/circuit ground jacks, Buz-1, Speed Test Input BNC, Test SYNC BNC, Gain and Filter Telegraph BNC'S, and the power cord interlock.
	Specifications
	Noise: (PC-ONE-40 10GOhm headstage) 1.0kHz Bandwidth, 0.06pA rms; 3.0kHz Bandwidth, 0.10pA rms; 5.0kHz Bandwidth, 0.16pA rms; 10kHz Bandwidth, 0.30pA rms Capacity Compensation: Fast: 0 to 1µs Slow: 0 to 1ms (Patch) Range: 10pF Patch; 100pF Whole Cell; 500pF Bilayer Series Resistance Compensation: 0 to 100MOhm, ten turn dial (all whole cell ranges) Filter: 100, 300, 1k, 3k, 10kHz Low Pass Bessel Filter (3 pole) Gain: 0.1, 0.2, 0.5, 1, 2, 5, 10, 20 amplification (b) of Im signal with headstage scale factor (a) of 0.1, 1, 10, 100 for sensitivities up to 2000mV/pA (Patch) (Scale factor (a) is shown on convenient LED's next to the GAIN switch) Junction Potential Correction: (3 types, all +300mV) Manual-ten turn control; Search continuously corrects potential with a slow "attack" time; Hold correction potential only when UPDATE switch is pushed TEST Commands: TEST switch with 0.2mV and 20mV positions for pipette and seal resistance forming seal (2pA and 200pA Current Clamp) Holding Potential Command: +200mV ten turn control with ON/OFF switch (+2nA Current Clamp) Panel Meter: 3-1/2 digit LCD with 5 functions and automatic decimal point - COMD In - the command at the command input (1999mV) - Vhold - the holding potential (1999mV) - Vtot comd - the total of all commands presented to the pipette (1999mv) - Vm - the membrane voltage (1999mV) - Im - the membrane current before processing: 199.9pA 10GOhm headstage; 19.99pA 100MOhm headstage	Step Command Input: 50:1 (i.e., 10 volts gives 200mV) industry standard, and 10:1 with center OFF (10kOhm input, 10 volts max) (5V/nA or 1V/nA Current Clamp) High Frequency Boost: Automatic with no need for confusing ON/OFF switch Computer Telegraph BNC Outputs: GAIN and FILTER, analog 0.4 volts per step External Mode Select: From Izero Mode, 5 volt CMOS input allows either Vclamp or Iclamp selection via I/0 15pin connector Membrane Current (Im) Output: Im:Unfiltered, Im signal. Sensitivity=(a)(b)mV/pA (used to record data unfiltered) FILTERED Im: Filtered lm signal. Sensitivity = (a)(b)mV/pA (used to view data) Sample & Hold Option: TTL activated Sample & Hold on Im path via back panel BNC Electrode Holders: (one supplied) Low noise polycarbonate with 1mm pressure port and BNC sized (ie., industry standard) end. Specify for glass size (HB180 is standard): HB120-accepts glass 1.0 to 1.2mm OD; HB180accepts glass 1.5 to 1.8mm OD (silver wire internal) Headstage Dimensions: 7.7cm x 3.5cm x 2.8cm ; detachable mounting rod is 0.62cm diameter by 7.6cm long. Cable length is 1.8m Mainframe Dimensions: 9cm high, 48cm wide, 25cm deep. Supplied with rack mounting hardware Power: 100/115 or 230 V/AC 50/60Hz (15W)

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