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Model
2400 |
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Patch
Clamp Amplifier
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A-M
Systems |
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The
Model 2400 is a low noise, full featured intracellular/extracellular
amplifier designed for voltage clamping using patch electrodes
on single channels or whole cells. Its unique design allows fast
intracellular current clamp measurements with sharp electrodes.
Advanced circuit design techniques using field programmable gate
arrays eliminate noisy microprocessors.
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Features
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Full-featured
voltage clamp with true current clamp capabilities
Voltage
clamp mode for both whole cell and patches
Current
clamp mode is 100% stable with bandwidth of 200kHz
Capacity,
series resistance, and whole cell compensation
4-pole low pass Bessel filter
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Internally
generated test signals
Telegraph
outputs for all major front panel controls
Independent
hold, offset, and tracking circuitry
Displays
command potentials, cell currents, and voltages
Dual
range low noise resistive feedback head stage
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Specifications
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Probe
Gain and Bandwidth
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Feedback Resistor
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Probe Gain
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Maximum Current
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Minimum Bandwidth
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10MΩ
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10mV/nA
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1000nA
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100kHz
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50MΩ
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50mV/nA
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200nA
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100kHz
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100MΩ
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100mV/nA
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100nA
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100kHz
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500MΩ
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500mV/nA
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20nA
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80kHz
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1GΩ
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1mV/pA
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10nA
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60kHz
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5GΩ
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5mV/pA
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2nA
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50kHz
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10GΩ
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10mV/pA
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1nA
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40kHz
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50GΩ
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50mV/pA
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0.2nA
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20kHz
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BNC
input connector: 1mm
ground pin connector
The
probe has three connections, the input BNC, a ground jack, and a
guard jack.
The
input BNC will hold any BNC style electrode holder, and is where
any input signal should be connected. The center conductor is
connected through a current limiting resistor to a field effect
transistor amplifier. Other than the current limiting resistor
there is no other protection to the probe so care must be taken to
avoid electrostatic discharges. The outer conductor of the BNC is
connected to a guard circuit. The guard is a buffered version of
the input voltage, so care must be taken not to ground the outer
conductor of the BNC.
Probe
Case Size 54mm long x 20mm high x 36mm wide
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Maximum
Instrument Noise
Voltage
clamp modes measured with the internal 4 pole Bessel filter and
minimum external noise
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Bandwidth
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Headstage
Resistor
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10MΩ
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50MΩ
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100MΩ
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500MΩ
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1GΩ
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5GΩ
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10GΩ
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50GΩ
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1kHz
Theoretical Limit
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0.002nA
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0.6
pA
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0.4
pA
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0.2
pA
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0.1
pA
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0.06
pA
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0.04
pA
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0.02
pA
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1kHz
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0.003nA
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0.7
pA
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0.5
pA
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0.3
pA
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0.17
pA
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0.10
pA
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0.08
pA
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0.06
pA
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Headstage
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The probe is both a current to voltage converter and a voltage
follower. The current to voltage converter used in voltage
clamping has one of two high value resistors used for amplifying
membrane currents. Below is a table of the different probes you
can purchase from A-M Systems and their feedback resistors.
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Catalog
Number
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Probe
Low Gain
Resistor Value (Ωs)
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Maximum
Current
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Probe
High Gain
Resistor Value (Ωs)
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Maximum
Current
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880210
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10M
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1000nA
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1G
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10nA
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880214
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50M
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200nA
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5G
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2nA
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880218
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10M
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1000nA
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10G
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1nA
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880222*
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100M
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100nA
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10G
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1nA
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880226
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50M
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200nA
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50G
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0.2nA
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880230
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500M
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20nA
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50G
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0.2nA
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*
Standard Headstage delivered with Model 2400 Patch Clamp
Amplifier unless one of the other Catalog Numbers is selected.
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Amplifiers
Products
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