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| Extracellular, Intracellular and Patch Clamp Amplifier |
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The
ELC-03XS amplifier is the only commercially available
amplifier that is not only designed for the
loose-patch clamp technique for recording in slice or
in vivo preparations, but also for a wide range of
other electrophysiological experiments. This amplifier
enables an investigator to identify a single cell in a
preparation by its characteristic spike train, then
inject dye, electroporate or stimulate this cell in
situ. The unique ELC headstage that meets these
diverse experimental requirements incorporates a
resistive-feedback circuit capable of functioning as a
patch clamp headstage with pA sensitivity, or as a
high-impedance electrometer with bridge balance
capable of functioning as a conventional bridge
amplifier, or as an extracellular amplifier with
differential input for sensitive measurement of field
potentials or single-unit activity. The ELC-03XS
amplifier offers an unprecedented combination of
capabilities and is much better suited for experiments
requiring multiple electrical recording or stimulation
protocols than other commercial amplifiers that are
designed specifically to perform only a single
function. Investigators with more limited equipment
budgets will also be pleased by the reasonable cost of
this versatile instrument. The ELC amplifier combines
the traditional approaches of electrical measurements,
electrical stimulation and dye application with new
approaches that make possible experiments under more
natural, i.e. non-invasive conditions, with minimal
damage to sensitive preparations, even on the level of
individual cells or subcellular structures such as
dendrites and axons. |
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The system includes a voltage clamp mode for
approaching cells and a current clamp mode for
recording. In current clamp mode a bridge balance
cancels electrode artifacts, and a current pump allows
electroporetic dye injection or transfection, if
required in VCx10 or CCx10 mode. Two digital displays,
an audio monitor, an electrode resistance test and
other functions make these amplifiers ideal for
extracellular loose-patch experiments. Additional
features are ten-turn controls for BRIDGE balance (CC)
and RS COMPENSATION (VC in patch clamp), Low-pass
Bessel filters for current and potential outputs,
highpass filter and variable gain on potential output
for extracellular recording. An oszillation shut off
unit and tunable penetration help complete this
instrument. |
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Specifications |
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HEADSTAGE: Input voltage range: ±1000 mV, other ranges possible Operating voltage: ±15 V Enclosure: Size: 23 x 70 x 26 mm, grounded Mounting plate: Size: 68 mm x 54 mm On request: Holding bar: length 150 mm, O.D. 8 mm Electrode connector: BNC with driven shield Ground connector: 2.4 mm connector Input resistance: >10E13 Ohm (internally adjustable) Current range: ±12 nA into 100 MOhm, other ranges possible ELECTRODE PARAMETER CONTROLS: OFFSET: range ±100 mV, ten-turn control CAPACITY COMPENSATION: range 0 - 30 pF, ten-turn control BIAS (internal adjustment): range ±100 pA, current adjustable with trim potentiometer BRIDGE BALANCE: 0-100 MOhm (0-1000 MOhm possible): Adjustable with ten-turn control ELECTRODE RESISTANCE TEST: Sensitivity 100 mV / MOhm application of square current pulses ±1 nA Display: 3 1/2 digit, XXX MOhm, activated by push-button (same as POTENTIAL display) BANDWIDTH AND SPEED RESPONSE: Full power bandwidth (REL = 0): >30 kHz, rise time (10% - 90%) <10 µs (REL = 100 MOhm) <5 µs (REL = 5 MOhm) DIGITAL DISPLAYS: Display mV / MOhm: 3 ½ digits, XXXX mV or XXX.X MOhm Display current: 3 ½ digits, XX.XX nA
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